countess blackboard subroutine

by Prof. Damion Weissnat DDS 7 min read

Illumina NovaSeq 6000 Sequencing System

The NovaSeq 6000 is Illumina’s latest advancement in ultra-high throughput NGS sequencing instrumentation. The NovaSeq is a dual flow cell instrument that is engineered to operate with 4 patterned flow cell formats providing 0.08 Tb – 6 Tb of total sequence data output.

10X Genomics Chromium Controller

The 10X Chromium Controller is a compact benchtop instrument that performs the encapsulation of individual cells or genomic DNA into 100K to 1M uniquely addressable partitions, each containing an identifying barcode for downstream genomic analysis.

Countess II FL Automated Cell Counter

Accurate cell counts and cell viability assessment is critical to the success of the Chromium Controller's operation. The Countess II FL Automated Cell Counter uses state-of-the-art optics and image analysis algorithms to analyze trypan blue stained cells in suspension.

Support Instrumentation

Additional equipment in support of the sequencing library preparation, validation and quantification includes the Covaris S2 Utrasonicator, Sage Science Pippin Prep preparative gel electrophoresis system, Agilent TapeStation 4200 and a Roche LightCycler96 Real Time PCR system.